DESKGEN Tile Library

Saturate coding and noncoding DNA with indels.
Illuminate genotype-phenotype relationships

DESKGEN Tile CRISPR Libraries are ideal for revealing and validating key functional domains, druggable targets, regulatory elements, and non-coding RNAs with saturated in situ mutagenesis.


Greater coverage - more discovery

DESKGEN Tile Libraries are AI-designed to make use of multiple nucleases and other techniques that provide higher coverage across any region of the genome.

With DESKGEN Tile, you can resolve functional domains, promoters, transcription factor binding sites and druggable targets with greater clarity.


Tile library customization

Each DESKGEN Tile library is built according to your model and delivery method and can be supplemented with additional nucleases - including those developed in your own lab.

Customize your Tile Library
Parameter
Customizable?
Example
Vector Yes Lentivirus
Target type Yes Super-enhancer
Number of targets Yes 150
Model organism Yes Mouse
Number of nuclease Yes Multiple
Nuclease type Yes SpCas9 & Cpf1

A Case Study

How functional genomics researchers used a DESKGEN Tile Library.
Understanding our client

A drug discovery group was interested in interrogating noncoding DNA using CRISPR.

They needed a high level of coverage to ensure nucleotide-level resolution but their off-the-shelf genome-scale SpCas9 CRISPR library could not met this requirement.


Five nucleases, double coverage

To achieve their goal, we included five different CRISPR nucleases to increase PAM site flexibility and coverage across their target regions. By adopting five different nucleases, we improved coverage by 2.1X compared with an SpCas9-only approach.

DESKGEN Tile Library
Parameter
Approach
Library type Pooled
Genome Human (GRCh38)
Nucleases SpCas9 | SaCas9 | St1Cas9
NmCas9 | AsCpf1
PAM coverage NGG | NNGRRT | NNAGAAW
NNNNGMTT | TTTV
Full documentation

To support further investigation, we provided the researchers with a final design report and manifest of all guides used in the experiment and their precise location in the target region.



Manufactured and delivered ready to use

The library was synthesized as oligos, cloned into lentiviral plasmids and quality controlled with NGS so our client could get straight to work.


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